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Jaebum Choo: Development of SERS-based assay platforms for highly sensitive in vitro diagnostics
发布日期:2017-06-18  字号:   【打印

报告时间2017年06月21日(星期三)09:00-10:00

报告地点:校学术会议中心二楼小报告厅

  Jaebum Choo 教授

工作单位韩国 汉阳大学生物纳米工程系

举办单位:仪器科学与光电工程学院

报告人简介

Jaebum Choo(朱宰范)教授于1994年在美国Texas A&M University获得博士学位,1994-1995年在加拿大University of Toronto进行博士后研究工作。1995年至今在韩国汉阳大学(Hanyang University)工作,目前是汉阳大学白南特聘讲座教授,担任韩国科技部BK-21 Plus生物纳米技术部部长,汉阳大学工学院研究副院长,曾任韩国集成人类传感系统国家工程中心(ERC)主任,韩国生物芯片学会主席,韩国科技部基础研究司评议委员,韩国研究基金委(NRF) 生命学部评议委员等职务。同时兼任Journal of Molecular Structure, Journal of Optical Society of Korea等期刊编委。近年来在微流控芯片,超灵敏医学传感、纳米材料、光谱仪小型化等基础理论研究及应用研究方面取得了突出成果,在包括ACS Nano, Chem. Soc. Rev., Lab Chip, Nano Lett., Biosens. Bioelectron., J. Am. Chem. Soc.等著名期刊上发表SCI论文240余篇,被引用7700余次,H指数46。国际会议邀请报告95次,国内会议邀请报告120余次,出版英文书籍5本。申请包括美国、日本、韩国专利32项,其中授权15项,并且3项专利技术已经在韩国进行产业转化。主持和参与了韩国贸易产业能源部(MOTIE)、科技部(MSIP)重大课题十余项。 

报告简介

Lateral flow assay (LFA) strip is a simple device intended to detect the presence of a target biomarker in clinical fluids. The benefits of LFA strip include a short time to obtain test result, a user-friendly format, low cost, and long-term stability. However, it has major limitations in terms of quantitative analysis and detection sensitivity. To resolve these problems, many different optical systems including fluorescence, chemi-luminescence and electrochemical readers have been employed but they still suffer from poor sensitivity and low precision. Our research group recently developed a novel surface-enhanced Raman scattering (SERS)-based assay platform for the highly sensitive biomarker detection. We believe that our proposed assay platform shows a significant improvement in the limit of detection (LOD) and multiplex detection capability for important clinical biomarkers. In this presentation, the current advances of SERS-based assay platforms and their clinical applications will be discussed. In addition, the integration of SERS with microfluidic platforms offers significant utility in chemical and biological experimentation. Herein, we report a fully integrated SERS-based microdroplet platform for the automatic immunoassay of specific target antigens. Specifically, highly efficient and rapid immunoreactions are achieved through sequential droplet generation, transport and merging, whilst wash-free immunodetection is realized through droplet-splitting. Such integration affords a novel multifunctional platform capable of performing complex multi-step immunoassays in nL-volume droplets. This assay system has additional advantages including reduced sample consumption (less than 100 μL), rapid assay times (less than 10 minutes) and fully automated fluid control. We anticipate that this integrated SERS-based microdroplet device provides new insights in the development of facile assay platforms for various hazardous materials.

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